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Method Validation In-Vitro Bioanalysis Extraction Human Plasma Fluorescence Detection Quantitation Acid Derivatization Liquid-Chromatography Mass Spectrometry HPLC Validation Separation Performance Liquid-Chromatography Plasma Mass-Spectrometry Electrospray-Ionization Tandem Mass-Spectrometry Quantification Chromatography Assay Rat Plasma Metabolites Pharmacokinetics LC-MS/MS Identification Solid-Phase Extraction Gas-Chromatography Urine